Fig. 5

CNS accumulation of αSyn inclusion pathology in APOE ε4^+/+/M83^+/− and APOE ε3^+/+/M83^+/− mice after intramuscular inoculation with PFFs. (a) Representative images of immunostaining with an antibody specific for αSyn phosphorylated at serine 129 (81A). Brains and spinal cord from APOE ε3^+/+/M83^+/− and APOE ε4^+/+/M83^+/− mice were assessed for relative pathological burden in M83^+/−mice. Scale bar = 100 μm. (b) Semi-quantification of 81A (pSer129) immunoreactivity in the spinal cord, medulla, pons, midbrain, hypothalamus, thalamus, cortex and striatum of PFF-injected M83^+/−, APOE ε4^+/+/M83^+/− and APOE ε3^+/+/M83^+/− mice were scored by 3 independent observers. Results were analyzed using 1-way ANOVA and corrected for multiple comparisons using Tukey’s test. Data represents means +/− SEM*p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001. (c) Kaplan–Meier analysis of motor phenotype development in PFF-injected M83^+/− (n = 8; median survival, 123 days after injection), APOE ε3^+/−/M83^+/− (n = 12; median survival, 112 days after injection), and APOE ε4^+/−/M83^+/− mice (n = 12; median survival, 115 days after injection) see Table 2. Overall Log-rank (Mantel–Cox) p = 0.4904