Fig. 4

Accumulation of αSynΔC in the midbrain of end stage TgM83^+/− mice after peripheral intramuscular PFF induction. (a) Nissl (left) and anatomical annotations (right) of coronal section panels from the Allen reference atlas of the adult mouse brain, midbrain regions analyzed in study are annotated in pink. (b, c) Representative IHC images and semi-quantification of antibody immunoreactivity comparing the pathological αSyn deposition of TgM83^+/− mice at end stage. Antibodies specific for αSyn phosphorylated at Ser129 (81A) and αSynΔC truncated at 103 (2G5), 114 (1A2), 122 (10A4), 125 (5C1) and 129 (2G7) were used for IHC, as indicated. Sections were counterstained with hematoxylin. Scale bar = 100 μm. Data expressed as mean +/− SEM. Results were analyzed using 2-way ANOVA and corrected for multiple comparisons using Tukey’s test. n = 8 (4F:4M); 9 (5F:4M); 8 (4F:4M); 8 (5F:3M). *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001