Fig. 3

Detection of αSynΔC truncated at residues 103 and 125 in the hindbrain of TgM83^+/− mice 2 months after peripheral intramuscular PFF inoculation. Representative IHC images and semiquantitative analysis comparing pathological αSyn deposition in TgM83^+/− mice at 1-, 2-, 3- months post IM injection or terminal stage in (a, b) the medulla and (c–e) the pons. Antibodies specific for αSyn phosphorylated at Ser129 (81A) and αSynΔC truncated at residues 103 (2G5), 114 (1A2), 122 (10A4), 125 (5C1) and 129 (2G7) were used for IHC, as indicated. Sections were counterstained with hematoxylin. c Nissl (left) and anatomical annotations (right) of coronal section panels from the Allen reference atlas of the adult mouse brain, pons regions analyzed in study are annotated in salmon. Scale bar = 1 mm for low magnitude image and 30 μm for inset in (a) and 60 μm in (d). Data represents mean +/− SEM. Results were analyzed using 2-way ANOVA and corrected for multiple comparisons using Tukey’s test. n = 8 (4F:4M); 9 (5F:4M); 8 (4F:4M); 8 (5F:3M). *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001