Fig. 2

Progressive accumulation of αSyn pathology and αSynΔC forms in the spine of TgM83^+/− mice following peripheral intramuscular administration of PFFs. (a) Representative IHC images comparing αSyn pathological deposition in TgM83^+/− mice at 1-, 2-, or 3- months post IM injection or terminal stage. Antibodies specific for αSyn phosphorylated at Ser129 (81A) and αSynΔC at residues 103 (2G5), 114 (1A2), 122 (10A4), 125 (5C1) and 129 (2G7) were used for IHC, as indicated. Sections were counterstained with hematoxylin. Scale bar for high magnification images = 150 μm; for inset = 15 μm. (b) Semi-quantification of antibody immunoreactivity for αSyn inclusion pathology. Data expressed as mean +/− SEM. Results were analyzed using 2-way ANOVA and corrected for multiple comparisons using Tukey’s test. n = 8 (4F:4M); 9 (5F:4M); 8 (4F:4M); 8 (5F:3M). *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001