Fig. 6

Ibrutinib treatment in Biozzi mice reduces CNS-infiltrating myeloid cell numbers and alters myeloid cell expression of activation markers. a, b Analyses were performed on n = 6 representative mice/group from the experiment shown in Fig. 4a. a Proportions of CNS-infiltrating lymphocytes that were CD4 T or B cells. b Numbers of CNS-infiltrating CD4 T or B cells. c-f Analyses were performed simultaneously on n = 6 age-matched naïve mice and n = 11–12 (all surviving) mice/group from the experiment shown in Fig. 4e. c Proportions of live cells in spinal cords that were IMCs or microglia. d Numbers of spinal cord IMCs or microglia. e, f Median fluorescence intensity (MFI) of activation markers in spinal cord IMCs or microglia. g-j Analyses were performed on n = 12 representative mice/group from the experiment shown in Fig. 4a. g Illustrative cervical spinal cord sections stained with anti-Iba1 antibody (brown) and hematoxylin (nuclei; blue). Magnified insets demonstrate Iba1⁺ areas in white matter. Scale bars, 100 µm. h Mean proportions of Iba1⁺ areas in whole spinal cord sections per mouse. i Illustrative cervical spinal cord white matter stained with anti-SMI32 antibody (brown) and hematoxylin (nuclei; blue). Both images are from the same white matter area (ventrolateral). Scale bars, 100 µm. j Mean proportions of SMI32⁺ areas in spinal cord white matter per mouse. Adjustments to input levels were made evenly across images in g, and evenly across images in i, to better visualize staining. Data in h and j were obtained by taking the mean value of 3 spinal cord Sects. (1 each of cervical, thoracic, and lumbar regions) per stain, per mouse. Graphed data are shown as mean ± SEM. Significance was tested using an unpaired two-tailed t test (a, b, h, and j) or one-way ANOVA followed by Dunnett's multiple comparisons test (c–f). *p < 0.050, **p < 0.010, ***p < 0.001