Fig. 2
Images of blood vessels labeled with tomato lectin649 (green) in cleared and labeled PLP-fixed human DLF tissue. a 200 µm thick DLF tissue section after clearing and staining with tomato lectin649, imaged at 4x. Overlaid squares represent the areas sampled per case: gray matter crest (red), white matter crest (white), gray matter sulcus (red dashed), and white matter sulcus (white dashed). 20 × images of each region are shown at top, dimension of each 347 × 336 × 91 µm. b 20 × image of a branching blood vessel and 60 × optical slices taken at 9 µm (c), 27.6 µm (d), and 35.4 µm into the tissue slice (e). f and g both show 20 × images of passively cleared and tomato lectin649-stained DLF tissue from different cases in the gray matter sulcus. A CTE case with high blood vessel fraction volume and branch density is shown in (f), with fraction volumes of 0.10 and and branch densitiy of 1500 branches/mm3. g shows an image stack from a non-CTEcontrol with lower vessel fraction volume (of 0.069) and branch density (980 branches/mm3). Each image stack is 81 µm thick