Fig. 5

Potent ITGA5 inhibitor, dioscin, demonstrates efficacy against characteristic cancer stem cell properties. Inhibitory effect on (A) proliferation, (B) self-renewing potential, and (C-E) frequency of self-renewing cells in recurrent Group 3 MB cells. Bars represent mean of at least three technical replicates. Bars represent mean of at least three technical replicates. *p ≤ 0.05, **p ≤ 0.001, ***p ≤ 0.0001, ****p ≤ 0.00001; unpaired t-test or one-way ANOVA with Sidak’s method for multiple comparisons