Fig. 3
Microglia number and function are unaltered in the PVN of T2DM individuals. a Representative images of Iba1-ir in the PVN of control (n = 20) and T2DM (n = 26) subjects. Plot of microglial parameters b Iba1-ir soma number/mm2 (cell density), c Iba1-ir average soma size and d relative masked area by the Iba1-ir microglial cells. e Co-localization of CD68 (a phagosome indicator) and Iba1 in the PVN and f Quantitative analysis of CD68-ir positive microglia in percentage and g volume percentage of CD68-ir in relation to Iba1-ir. h Co-labelling of Oxt and Iba1 in the PVN and mean relative area masked (%) of Iba1-ir particles surrounding Oxt-ir neurons within 10 µm radius (i). j Co-labelling of AVP and Iba1 in the PVN and k mean relative area of coverage (%) of Iba1-ir particles surrounding AVP-ir neurons within 10 µm radius. Scale bar: 20 µm in a; 10 µm in e and 8 µm in h and j. Data are represented as mean ± SEM. *p < 0.05. Significance was calculated using Student`s t test in b–d, f, g, i, k