Fig. 9

Locomotor activity in mice following single or r-mTBI. Locomotor activity following five (A, C, E and G) and one (B, D, F and H) impact at 1, 3, 6, 9 and 12 months post-injury . There were no significant changes in all four parameters between TBI and Sham animals across both injury groups. On average (not significant), A 5x TBI animals spent more time being ambulatory than Shams, B 1x TBI animals spent less time being ambulatory than Shams, C 5x TBI animals made more zone entries than Shams, D 1x TBI animals made less zone entries than Shams, E and F no major differences in resting time for 5x and 1x TBI animals compared to their respective Shams, G 5x TBI animals made more vertical counts than Shams, (H) 1x TBI animals on average made less vertical counts up to 6 months which was reversed up to 12 months post-injury. Mixed effects analysis with Holm-Sidak post-hoc for all graphs except for (D and F) which were analysed by two-way repeated measures ANOVA due to no missing values. Data expressed as mean ± SEM, *P < 0.05, **P < 0.01, n = 8–10/group, * indicates a significant difference within Sham animals across time points