Fig. 8

Neurological status in mice following single or r-mTBI. Assessment of neurological status using the mNSS test following five (A) and one (B) impact at 1, 3, 6, 9 and 12 months post-injury. (A) Significant increase in mNSS scores following five impacts compared to Shams at 1-month post-injury, with no differences thereafter. (B) No significant differences in mNSS scores following one impact across all time points. Two separate cohorts of animals were used at 1–6 and 9–12 months post-injury for both injury groups, represented by a gap on the x-axis. A Two-way repeated measures ANOVA (1–6 months) and a mixed-effects analysis (9–12 months) were conducted separately for each cohort. Because both cohorts followed similar trends, the data are presented on the same graph solely for illustrative purposes. Note that the 1-month time point presented in this figure has been previously published elsewhere [44] and that the authors have obtained permission to re-use this data. Data expressed as mean ± SEM, #P < 0.05, ##p < 0.01, ****P < 0.0001, n = 7–10 (Sham), n = 9–10 (TBI), * indicates a significant difference between Sham and TBI animals, # indicates a significant difference within Sham animals across time points