Fig. 7
Expression of iron-regulatory proteins and total iron content in mice following single or r-mTBI. Iron-regulatory proteins (A-I) and total iron content (J and K) in the ipsilateral and contralateral hemispheres of the parietal cortex at 12 months post-injury. (A-II to VII) Following five impacts, there were no significant changes in the expression of any proteins of interest at 12 months post-injury despite a slight trend towards increased Pin1 in the ipsilateral insoluble fraction (A-III). B-II to VII Following one impact, there were no significant changes in the expression of any proteins at 12 months post-injury, despite a trend towards increased levels of PP2A-a subunit (B-V) in the contralateral insoluble fraction. Representative western blot images (A-I and B-I) correspond to the hemisphere and fraction of the protein depicted in the graph. Example of a loading control blot for PME1 from the 5 hit group (A-I) and the 1 hit group (B-I). Note that each of the antibodies were run on separate blots but are represented herein as a composite image for the purpose of clarity. Unpaired two-tailed Student’s t-test. Data expressed as mean ± SEM, *P < 0.05, n = 7 (Sham), n = 7–8 (TBI)