Fig. 6

Expression profile of neurodegeneration-associated proteins in mice following single or r-mTBI. Western blots in the parietal cortex following five (A) and one (B) impact at 12 months post-injury. A-I to A-VII Following five impacts, there were no significant changes in the expression of any proteins of interest at 12 months post-injury. B-III Following one impact, there was a significant increase in T22 oligomeric tau and (B-VI) a trend towards increased Thr231 in the soluble fraction of the contralateral hemisphere at 12 months post-injury. B-II, IV, V and VII There were no other changes in the expression of other proteins of interest at 12 months post-injury. Representative western blot images (A-I and B-I) correspond to the hemisphere and fraction of the protein depicted in the graph. Example of a loading control blot for Total tau from the 5 hit group (A-I) and the 1 hit group (B-I). All phospho-tau proteins are normalised to total protein content and total tau of each sample. Note that each of the antibodies were run on separate blots but are represented herein as a composite image for the purpose of clarity. Unpaired two-tailed Student’s t-test. Data expressed as mean ± SEM, *P < 0.05, n = 7 (Sham), n = 8 (TBI)