Fig. 1

Experimental design of the animal study. (A, B) Schematic representation of the protocol used to prepare postdependent animals and controls, including the chronic intermittent exposure (CIE) regime, respectively. (C) Schematic representation of the sequential procedures applied to each subject in the study. (D) Location of the recording (green) and stimulating (red) electrodes, respectively. Histological image (left) and corresponding atlas section (right) [48]. The yellow shading marks the targeted region. Final electrode positioning was always based on the recording of well-known pathway-specific evoked potentials. Arrows point to the DiI trace left by the recording electrode and the tissue damage produced by stimulating electrodes