Fig. 7

Attenuation of microglial response and myelin renewal are not impaired in Abcd1 KO mice. Representative images of the corpus callosum of WT and Abcd1 KO mice after cuprizone withdrawal showing immunohistochemical detection and quantification of (a, b) PLP⁺ myelin, (c, d) total (IBA1⁺) microglia and (e, f) activated (MAC3⁺) microglia at 5 days, 3 weeks and 7 weeks post cuprizone (CPZ). Scale bar: 100 µm. Two outliers detected by the Grubbs outlier test at 3 weeks off CPZ (one each from the PLP WT and MAC3 WT groups) are shown in the graphs but were excluded from the statistics. Statistical analysis: One-way ANOVA with Sidak’s multiple comparisons test (n = 6–10 WT; 6–8 Abcd1 KO mice). For MAC3 5 days off CPZ, adjusted p-value: *p = 0.0366