Fig. 5

Abcd1 KO OPCs proliferate normally in response to cuprizone intoxication. (a–d) Representative images of immunohistochemistry for OLIG2 in the corpus callosum of WT and Abcd1 KO mice before, during (a) and after (c) dietary cuprizone administration showing all cells of the oligodendrocyte lineage. Scale bar: 100 µm. Quantifications of OLIG2⁺ cell density (cells/mm²) in the demyelination (b) and remyelination (d) stages are depicted as box plots according to Tukey with dot plot overlays, medians and 1.5 IQR error bars. The data points represented in the micrographs are color-coded in black. Statistical analysis: One-way ANOVA with Sidak’s multiple comparisons test (n = 5–10 WT, 4–10 Abcd1 KO mice)