Fig. 4

WT and Abcd1 KO mice show similar extent of astrocyte activation during cuprizone-induced demyelination. (a) Representative immunohistochemistry images for GFAP in the medial corpus callosum of WT and Abcd1 KO mice at baseline, 3 weeks and 5 weeks of cuprizone treatment. Scale bar: 100 µm. (b) Quantification of GFAP staining is displayed as % of the total area analyzed. The data are depicted as box plots according to Tukey with dot plot overlays, medians and 1.5 IQR error bars. The data points represented in the micrographs are colod-coded in black. Statistical analysis: One-way ANOVA with Sidak’s multiple comparisons test. Controls: n = 5 WT, 4 Abcd1 KO; 3 weeks CPZ: n = 10 WT, 9 Abcd1 KO; 5 weeks CPZ: n = 10 WT, 8 Abcd1 KO