Fig. 3

WT and Abcd1 KO mice show similar dynamics of myelin loss and microglia activation during cuprizone-induced demyelination. (a–f) Representative images of the medial corpus callosum of WT and Abcd1 KO mice showing immunohistochemistry for the myelin protein PLP (a), total (IBA1⁺) microglia (c) and activated (MAC3⁺) microglia (e) and quantification at baseline, 3 weeks and 5 weeks of cuprizone treatment. Scale bar: 100 µm. Quantification of the PLP⁺ (b), IBA1⁺ (d) and MAC3⁺ (f) staining is displayed as % of total area analyzed. The data are shown as box plots according to Tukey with dot plot overlays, medians and 1.5 IQR error bars. The data points represented in the micrographs are color-coded in black. Statistical analysis: One-way ANOVA with Sidak’s multiple comparisons test. Controls: n = 5 WT, 4 Abcd1 KO; 3 weeks CPZ: n = 10 WT, 9 Abcd1 KO; 5 weeks CPZ: n = 10 WT, 8 Abcd1 KO