Fig. 2

Accelerated oligodendrocyte loss and axonopathy in cuprizone-intoxicated Abcd1 KO mice. (a) Representative images of CAII immunohistochemistry showing mature oligodendrocytes at control, 3 and 5 weeks cuprizone (CPZ) treatment in the medial corpus callosum of WT and Abcd1 KO mice. Scale bar: 100 µm. (b) Confocal triple staining showing mature oligodendrocytes (CAII, red), all cells of the oligodendrocytic lineage (Olig2, green) and astrocytes (GFAP, blue) at 3 weeks CPZ, WT and Abcd1 KO mice (scale bar: 20 µm). (c) Micrographs showing APP⁺ axonal spheroids revealing acute axonal damage as described in (a). Scale bar: 100 µm. Magnified view of the 3 weeks CPZ, Abcd1 KO; scale bar: 50 µm. (d, e) Quantifications of the number of CAII⁺ cells and APP⁺ spheroids are shown as box plots according to Tukey with dot plot overlays, medians and 1.5 IQR error bars. The data points represented in the micrographs are color-coded in black. Grubbs outlier test detected two outliers at 3 weeks CPZ for APP and two at 5 weeks CPZ for CAII, shown in the graphs, but excluded from the subsequent statistical analysis. Statistics: One-way ANOVA with Sidak’s multiple comparisons test. Controls: n = 5 WT, 4 Abcd1 KO; 3 weeks CPZ: n = 10 WT, 9 Abcd1 KO; 5 weeks CPZ: n = 10 WT, 8 Abcd1 KO. Adjusted p-value: *p = 0.0226; ****p < 0.0001)