Fig. 1

The significant decrease of hippocampal cholesterol level in 5xFAD mice. A Quantification of cholesterol level in hippocampus of WT and 5xFAD mice (3 month) by LC–MS/MS analysis. n = 3–4 sample/group, every sample was included 2–3 mice randomly. B–D The fold change expression of mRNA of DHCR24, HMGCR and SREBP2 in hippocampus of WT and 5xFAD mice (3 month) by RT-PCR. E The immunoblotting bands of DHCR24, HMGCR, SREBP2 in hippocampus of 3-month-old WT mice and 5xFAD mice. F Analysis of western blot with mean gray value which all were quantification on the ratio of target proteins against GAPDH. G The fluorescence images of DHCR24 (red) in hippocampus of 3-month-old WT and 5xFAD mice (scale bar, 20 μm). H Mean fluorescence intensity of DHCR24. n = 3 mice per group in [B-H]. Data expressed as mean ± SEM, statistical analysis was performed using unpaired two-tailed Student’s t-test. *P < 0.05; **P < 0.01; ***P < 0.001; compared with aged-matched WT group