Fig. 4
From: Astrocytic uptake of neuronal corpses promotes cell-to-cell spreading of tau pathology
Long-term Tau-F exposure of astrocytes affect their viability and reactivity. a Astrocytes exposed to Tau-F, co-labelled for LAMP-1, GFAP, Actin and DAPI. b Representative images of astrocytes exposed to Tau-F for 3 days, 14 days and 28 days, stained for GFAP, Actin and LAMP-1. c Quantification of total astrocyte number, average cell area and number of branch points per cell. d Quantification of IntDen/cell for GFAP. e Quantification of IntDen/cell for actin. f Quantification of IntDen/cell for LAMP-1. The control group is combined from all time point since there was no statistically significant variation over time. For GFAP images, scale bars in a, are set to 50 µm and in b, 100 µm, 50 µm. For actin and LAMP-1, the scale bars = 25 µm. Statistical analysis in c-f was performed using Kruskal–Wallis test. *p < 0.05, **p < 0.01, ***p < 0.005, ****p < 0.0001