Fig. 2
From: Astrocytic uptake of neuronal corpses promotes cell-to-cell spreading of tau pathology
Sonicated tau fibrils induce distinct tau pathology in human iPSC-derived neurons. a WB analysis of cell lysates from control, Tau-O and Tau-F exposed neurons for Gsk3-β, PP2A, AT8 and pSer400 (relative to total protein). b Representative fluorescence images of total tau (Tau-1) in control neurons and Tau-F exposed neurons. Tau-F exposed neurons display a distinct tau-pattern with spherical deposits. Scale bars = 200 µm. c Control and Tau-F exposed neurons stained for synaptophysin (co-staining with WGA is presented in Additional file 4: Fig. S3a). Scale bars = 50 µm. d Quantification of synaptophysin (Syn) positive puncta and TUNEL positive nuclei (representative images are shown in Additional file 4: Fig. S3b) normalized to the total number of cells. Error bars represent min/max values. One-way ANOVA and t-test were used for graphs in (a) and (d) respectively. *p < 0.05, **p < 0.01, ***p < 0.005, ****p < 0.0001