Fig. 5

Complement inhibition affects the abundance of brain resident immune cells and the expression of functional receptors on infiltrating cells in the injured hemisphere. A Number of immune cells per experimental group and sample as identified by the expression of CD45 in the CyTOF panel. Data was acquired from ~ 62,000 cells from the injured hemisphere. The number of immune cells shown is normalized by the total number of isolated cells (see methods). B Summary of the change in percentage and the fold change in cell count of each immune cell type at days 3, 7 and 28 after TBI compared to the sham group. Non-significant changes are in gray. C MDS plot summarizing the differences in functional receptor expression among all 42 samples. The circle size is proportional to the number of immune cells in the sample. D, E Summary of all the significant changes in the expression of functional receptors by immune cell types D and percentage of functional clusters E upon treatment with CR2-Crry. Data are shown as mean and color-coded for statistical significance in panels B, D and E. N = 6 per group. *p < 0.05. Bonferroni correction was used for multiple comparisons in panel A. False discovery rate was used to adjust the p-value in the other panels. Abbreviations: cDC = conventional dendritic cell, pDC = plasmacytoid dendritic cell, MdC = monocyte-derived cell