Fig. 7

Comparative analysis of Aβ, tau and α-synuclein in AD patients. Forebrains of AD patients were stratified into E4 and non-E4 genotypes and stained for total Aβ (A), AT8-immunoreactive phospho-tau (B) and total α-synuclein (C). Brains of age-matched healthy controls used were all non-E4 genotype. Representative images of immunostained sections and corresponding quantitation of immunostaining shows increased Aβ, tau and α-synuclein in AD patients. Scale Bar, 150 µm; 1-way Anova with Sidak’s multiple comparison test. ***p < 0.0001; ***p < 0.001; **p < 0.01; *p < 0.05. Open circle, control non-dementia healthy cases; closed circles, pure AD cases and open diamonds, mixed AD pathology cases. (D) Linear covariance analysis of Aβ burden and tau burden in AD patients stratified into E4 or non-E4 genotype. (E) Linear covariance analysis of α-synuclein burden and tau burden in AD patients stratified into E4 or non-E4 genotype. N = 15–18 healthy controls, n = 20–21 AD (non-E4) and n = 23 (E4). Pearson’s correlation coefficient, r, and p value of correlation indicated on corresponding graphs