Fig. 1

APOE isoform influences survival and tauopathy burden in PS19 mice. PS19 mice homozygous for APOE allele were aged until they exhibited bilateral hindlimb paralysis. A The percentage of surviving mice was plotted against survival days for each APOE genotype (blue circle = PS/E2H; purple square = PS/E3H; red triangle = PS/E4H; grey diamond = PS19). n = 10 mice/genotype (PS/E2H, PS/E3H), 18 mice (PS/E4H) and 16 mice (PS19). Log-rank (Mantel-Cox) test. B Immunoblotting of total tau was done in detergent soluble (S1) and sarkosyl-insoluble (S3 pellet) brain fractions derived from paralyzed mice. Band intensity of S1-total tau was normalized to GAPDH, whereas band intensity of S3-total tau was normalized to the total protein concentration of the brain homogenates. Numerals on the left side of each blot denote molecular weight standards in kDa. n = 3 mice/genotype. C Paralyzed PS/E2H, PS/E3H and PS/E4H mice were analyzed for phospho-tau levels using AT8 antibody. Representative immuno-stained images and corresponding quantification of AT8 immunostaining (% immunoreactivity) in the cortex and hippocampus are shown. n = 9 (PS/E2H), 7 (PS/E3H), 16 (PS/E4H).1-way ANOVA *p < 0.05, **p < 0.01. Scale bar: 100 µm. D Immunoblotting of AT8-tau was done in detergent soluble (S1) brain fraction derived from paralyzed mice. Band intensity of S1-total tau was normalized to GAPDH. Numerals on the left side of each blot denote molecular weight standards in kDa. n = 3 mice/genotype. 1-way Anova