Fig. 7

The pro-regenerative factors identified in monocyte-derived macrophages can promote axon regeneration via paracrine signalling. A Schematic overview of the experimental setup of the different recombinant protein mixes intravitreally injected in the eye. B Representative images of regenerating axons that were CTB-traced on longitudinal cryosections of the optic nerve of mice at 14 dpi ONC and intravitreally injected with PBS, mix 1, mix 2 and mix 1 + 2. The ONC site is indicated with an asterisk. Scale bar 100 µm. C Quantification of axonal regeneration on longitudinal cryosections of the optic nerve of mice at 14 dpi ONC and intravitreal injection of PBS, mix 1, mix 2 and mix 1 + 2. Axonal number was counted at various distances starting at 150 µm from the ONC lesion site. Data are shown as mean ± SEM. Repeated measures two-way ANOVA followed by Tukey’s multiple comparisons test, statistical significance between different conditions at the same distance is indicated with different letters: conditions that share the same letter are not significantly different, while conditions with different letters are significantly different from each other, n = 4–5 mice per condition