Fig. 2

Accelerated senescence enhances aberrant intracellular Aβ accumulation in 5xFAD mice. a Immunostaining analysis of Aβ42 (Aβ42 antibody, clone H31L21, red) in the subiculum from 1.5-month-old 5xFAD and G3Terc^−/− 5xFAD mice. Representative photomicrographs are shown. Quantitative analysis of intracellular Aβ42 was performed by counting cells displaying intracellular Aβ42 staining (arrows). Scale bar: 100 μm. ***P < 0.001 (two-tailed Student’s t-test, n = 5–7 mice/group). b Immunostaining analysis of Aβ42 and Aβ42-containing plaques (Aβ42 antibody, clone H31L21, red; Thioflavin T dye, green, colocalization indicated by arrows) in the subiculum from 2-month-old 5xFAD and G3Terc^−/− 5xFAD mice. Representative photomicrographs are shown. Scale bar: 100 μm. c Immunostaining analysis of Aβ42 (Aβ42 antibody, clone H31L21, red) in the cortical layer V region from 2-month-old 5xFAD and G3Terc^−/− 5xFAD mice. Representative photomicrographs are shown. Quantitative analysis of intracellular Aβ42 was performed by counting cells displaying intracellular Aβ42 staining (arrows). Scale bar: 200 μm. Non-significant (two-tailed Student’s t-test, n = 3–5 mice/group). All data are presented as the mean ± SEM