Fig. 8
Analysis of Aβ proteoforms in APP WT and APP FAD mutants by immunoprecipitation-mass spectrometry. IP-MALDI-TOF on A human CSF positive control, B conditioned media from wild type HEK293T cells and transiently transfected HEK293T cells with C Swedish, D Iberian, E Iowa, F Flemish and G Arctic mutations yielded mass spectra of Aβ proteoforms. Data from only one biological replicate (out of two) are shown. ¤ indicates [Aβ1-40 + 2H]2+ and * represent peaks with unknown identities. Peak identity is considered confirmed when the m/z corresponds to the theoretical m/z of an Aβ proteoforms with a tolerance of 20 ppm. In the inserts, the arrows point to the monoisotopic peak of some interesting proteoforms