Fig. 7

Analysis of the proteolytic processing of APP WT and APP FAD mutants. A HEK293T cells were transiently transfected with c-myc-tagged APP, Swedish, Flemish, Arctic, Iowa or Iberian. Equal volumes of cell lysates and conditioned media were analyzed via Western Blot. To detect APP full length protein, the cell lysate was analyzed with antibody 22C11. For detection of α-CTF and β-CTF, the lysate was examined with antibody Y188. The total amount of sAPP was detected with antibody 22C11 using conditioned media. The media was furthermore analyzed with antibodies W02 (to visualize sAPPα) and anti-sAPPβ. B–F Quantification of Western Blot signals from A. Normalized ratios from processed fragments to full length APP are shown (normalized to APP WT). Bars represent mean values ± SEM; n > 5 (5 biological replicates). Statistical analysis was performed using ANOVA test (*p < 0.05, **p < 0.01, ***p < 0.001)