Fig. 5

Colocalization of APP WT and APP FAD mutants in Early Endosomes. A HeLa cells were transiently transfected with N-terminally c-myc-tagged APP WT, Swedish, Arctic, Flemish, Iowa or Iberian. Cells were labeled with α-c-myc and α-EEA1 (marker for early endosomes) antibodies. The merge reveals colocalization of both channels, indicated in yellow. Scale bar: 20 μm. B ICC quantification. The relative mean integrated density indicates the percentage of APP present in Early Endosomes. Bars represent mean values ± SEM; n = 30 (three biological replicates). Statistical analysis was performed using one way ANOVA with Tukey`s post hoc test (*p < 0.05, **p < 0.01, ***p < 0.001)