Fig. 2

Analysis of dimer formation of APP WT and APP FAD mutants. A HEK293T cells were transiently transfected with N-terminally c-myc-tagged APP WT, Arctic, Flemish, Iberian, Iowa or Swedish. Cell lysates were prepared, and equal amounts of protein were analyzed via BN-PAGE and subsequent western blot detection. APP and the FAD mutants were detected with an anti-c-myc antibody. Full length APP and monomers/dimers are indicated with arrows. B BN-PAGE quantification. The relative dimer intensity represents the ratio of APP dimer intensity to the total APP signal intensity (APP dimer plus monomer). Bars represent mean values ± SEM; n = 3 (three biological replicates). Statistical analysis using one way ANOVA with Tukey`s post hoc test did not reveal significant differences