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Table 2 Antibodies used for Immunoblotting and Immunostaining

From: Patients with sporadic FTLD exhibit similar increases in lysosomal proteins and storage material as patients with FTD due to GRN mutations

Target

Application

Sample Type

Source/Cat. #

Dilution

CatD

IB

Patient

Santa Cruz Biotechnology, sc-

1:500

 

IF

 

6486

1:500

CatD

IB

Mouse

R&D Systems, AF1029

1:500

 

IF

  

1:1000

GCase

IB

Patient

MilliporeSigma, G4171

1:500

GCase

IF

Patient

R&D Systems, MAB7410

1:250

GFAP

IF

Patient/Mouse

Agilent, Z033429

1:1000

HexA

IB

Patient

Santa Cruz Biotechnology, sc-376777

1:500

HexA

IB

Mouse

Abcam, ab189865

1:500

Iba1

IF

Patient/Mouse

Wako, 019–19,741

1:500

LAMP-1

IB

Patient

Santa Cruz Biotechnology, sc-20011

1:1000

LAMP-1

IB

Mouse

Developmental Studies Hybridoma Bank, 1D4B

1:100

LAMP-2

IB

Patient

Santa Cruz Biotechnology, sc-18822

1:1000

MAP2

IF

Patient

ThermoFisher, PA1-10,005

1:1000

NeuN

IF

Patient

Abcam, ab177487

1:1000

NeuN

IF

Mouse

MilliporeSigma, Abn91

1:500

p-TDP-43 (Ser409/410)

IF

Mouse

Proteintech, 22,309–1-AP

1:1000

 

IHC

  

1:60,000

 

IB

Patient

 

1:500

SCMAS

IHC

Mouse

Abcam, ab181243

1:500

  1. CatD cathepsin D, GCase β-glucocerebrosidase, HexA β-hexosaminidase A, IB immunoblot, IF immunofluorescence, IHC immunohistochemistry, SCMAS subunit C of mitochondrial ATP synthase