Fig. 1From: Patients with sporadic FTLD exhibit similar increases in lysosomal proteins and storage material as patients with FTD due to GRN mutationsIncreases in lysosomal enzyme activity and protein levels in frontal cortex of patients with FTD-GRN and sporadic FTLD-TDP type A. a Analysis of lysates from frontal cortex of patients with FTD-GRN or sporadic FTLD-TDP type A revealed similar increases in HexA activity (ANOVA, p = 0.0171) and CatD activity (ANOVA, p = 0.0404) in each patient group. No changes in GCase activity were detected (ANOVA, p = 0.4053). b Lysosomal protein levels followed a similar pattern, with both patient groups exhibiting increased levels of mature CatD (ANOVA, p = 0.0429), LAMP-1 (ANOVA, p = 0.0170), and LAMP-2 (ANOVA, p = 0.0019). c In contrast, no significant changes in enzyme activity were detected in occipital cortex, though there was a trend for reduced GCase activity in FTD patients (ANOVA, p = 0.0595). d Levels of mature GCase protein were reduced in occipital cortex of both patient groups (ANOVA, p = 0.0159) and the only elevated lysosomal protein in occipital cortex was LAMP-2, which was only elevated in patients with FTD-GRN (ANOVA, p = 0.0232). Immunoblots for frontal cortex are shown in e and for occipital cortex are shown in f. HexA = β-hexosaminidase A, CatD = cathepsin D, GCase = β-glucocerebrosidase. In e, f Ct = control, G = FTD-GRN, and A = sporadic FTLD-TDP type A. Molecular weight markers are identified by weight in kDa for each blot. †p < 0.1, *p < 0.05, **p < 0.01, and ***p < 0.001 by Fisher’s LSD post-hoc test. n = 5 controls, 12 patients with FTD-GRN, and 7 patients with sporadic FTLD-TDP type ABack to article page