Fig. 6
G51D PD-associated α-syn aggregates are more stable than MSA-associated α-syn aggregates. a Representative immunoblots from conformational stability assay analysis of α-syn aggregates in brain homogenates from human G51D PD, sporadic PD, and MSA samples. b Denaturation curves for residual PSyn levels (mean ± s.e.m. of 3–4 technical replicates per sample) following treatment with the indicated concentrations of GdnHCl. The calculated [GdnHCl]50 values for each sample (± standard error) are shown. c Representative immunoblots from conformational stability assay analysis of α-syn aggregates in brain homogenates from asymptomatic G51D PD-1 mice and G51D PD-2 mice at 540–543 DPI, or from a clinically ill MSA mouse. d Denaturation curves for residual PSyn levels (mean ± s.e.m. from n = 3 mice per inoculum) following treatment with the indicated concentrations of GdnHCl. The calculated [GdnHCl]50 values for α-syn aggregates within each experimental group (± standard error) are shown. In panels a and c, detergent-insoluble PSyn was detected using the antibody EP1536Y