Fig. 5
G51D PD-derived α-syn aggregates possess seeding properties more reminiscent of sporadic PD than MSA. a ThT fluorescence curves for α-syn SAA experiments using a PD-enhanced buffer on α-syn aggregates from G51D PD, MSA, sporadic PD, and control human brain homogenates. Monomeric wild-type human α-syn was seeded with either G51D PD-1 (red), G51D PD-2 (orange), MSA-1 (black), MSA-2 (brown), sporadic PD-1 (dark blue), sporadic PD-2 (light blue), or control (green) brain extract. Each data point represents the mean ThT fluorescence ± s.e.m. from 4 to 6 technical replicates. b Maximum ThT fluorescence values obtained during α-syn SAA with the PD-enhanced buffer using brain homogenates from the indicated human samples as seeds. Data is mean ± s.e.m. from 4 to 6 technical replicates. c ThT fluorescence curves for α-syn SAA experiments using an MSA-enhanced buffer on α-syn aggregates from G51D PD, MSA, sporadic PD, and human control brain homogenates. Each data point represents the mean ThT fluorescence ± s.e.m. from 4 to 6 technical replicates. d Maximum ThT fluorescence values obtained during α-syn SAA with the MSA-enhanced buffer using brain homogenates from the indicated human samples as seeds. Data is mean ± s.e.m. from 4 to 6 technical replicates. e ThT fluorescence curves for α-syn SAA experiments using an MSA-enhanced buffer on α-syn aggregates in brain homogenates from aged uninoculated TgM83+/- mice (green, n = 10), G51D PD-1 mice at either 21 (pink, n = 9) or 543 (red, n = 6) DPI, G51D PD-2 mice at 540 DPI (orange, n = 5), or clinically ill MSA mice (black, n = 8). Data is mean ± s.e.m. from 3 to 4 technical replicates per mouse. f Maximum ThT fluorescence values obtained during α-syn SAA with the MSA-enhanced buffer using brain homogenates from TgM83+/- mice injected with the indicated human samples as seeds. Data is mean ± s.e.m. from 3 to 4 technical replicates per mouse. For panels b, d, and f, P values were calculated using a Brown-Forsythe ANOVA test followed by Dunnett’s T3 multiple comparisons test