Fig. 3

G51D PD and MSA produce distinct patterns of PSyn deposition in TgM83^+/- mice. a Images of PSyn-stained brain sections (EP1536Y antibody) from a clinically ill MSA mouse at 257 DPI and an asymptomatic G51D PD-1 mouse at 543 DPI. Colored boxes indicate areas of the brain shown in higher magnification in panel b. Scale bar = 1 mm (applies to both images). b Images of the midbrain (black border), hypothalamus (red border), and brainstem (blue border) from a clinically ill MSA mouse at 257 DPI as well as the brain base (black border) and parahippocampal region (red border) from an asymptomatic G51D PD-1 mouse at 543 DPI. All sections were stained with the EP1536Y PSyn antibody. Scale bar = 50 µm (applies to all images). c Quantification of PSyn deposition in the hypothalamus, midbrain, and brainstem of G51D PD-1 mice at 543 DPI (red, n = 6), G51D PD-2 mice at 540 DPI (orange, n = 5), or clinically ill MSA mice at 141–257 DPI (black, n = 6). Data is mean ± s.e.m. P values were calculated using a two-way ANOVA with Tukey’s multiple comparisons test. d Quantification of the number of PSyn deposits in the parahippocampal region (left) and brain base (right) of aged uninoculated TgM83^+/- mice (n = 10), G51D PD-1 mice at either 21 DPI (n = 9) or 543 DPI (n = 6 for parahippocampal, n = 5 for brain base), G51D PD-2 mice at 540 DPI (n = 5), and clinically ill MSA mice at 141–257 DPI (n = 6). Data is mean ± s.e.m. P values were calculated using a Kruskal–Wallis test followed by Dunn’s multiple comparisons test