Fig. 2

Biochemical detection of α-syn aggregates in the brains of inoculated TgM83^+/- mice. a Immunoblots of total α-syn, detergent-soluble α-syn, and detergent-insoluble PSyn species in brain homogenates from G51D PD-1 and G51D PD-2 mice at 540–543 DPI, clinically ill MSA mice at 155–257 DPI, as well as uninoculated TgM83^+/- mice at 580 days of age. The blot of total α-syn was reprobed with an antibody against actin. b Quantification of detergent-insoluble PSyn levels (mean ± s.e.m.) in the brains of G51D PD-1 (n = 6) and G51D PD-2 (n = 5) mice at 540–543 DPI compared to age-matched uninoculated TgM83^+/- mice (n = 8). P values were calculated using one-way ANOVA followed by Dunnett’s multiple comparisons test. c Immunoblot of detergent-insoluble, thermolysin (TL)-resistant PSyn and total α-syn levels in brain homogenates from G51D PD-1 mice at 543 DPI or age-matched uninoculated TgM83^+/- mice (n = 6 each). d Immunoblot of detergent-insoluble, TL-resistant PSyn levels in brain homogenates from G51D PD-1 mice at either 21 (n = 9) or 543 (n = 2) DPI. In panels a, c, and d, PSyn was detected using the antibody EP1536Y and α-syn was detected using the antibody Syn-1