Fig. 4

Immunohistopathological study with anti-GFAP antibody. Immunofluorescence study of retina and optic nerve with anti-GFAP antibody (red) and anti-ubiquitin antibody (green). A Merged view of Immunofluorescence stain of NIID retina (Case 1) with GFAP (B, red), with ubiquitin (C, green) and with DAPI (D, blue). Severe gliosis and ubiquitin-positive intranuclear inclusions were observed. E–H: The same Immunofluorescence stain of control retina. I–J Low magnification of NIID retina (I: Case 2) and control retina (J). K–L: Optic nerve of NIID (K: Case1) and control (L). Severe gliosis was observed in both retina and optic nerve of NIID cases. Scale bars: A–H and K–L: 10 μm, I–J: 20 μm