Fig. 7
From: ER stress induced immunopathology involving complement in CADASIL: implications for therapeutics
Complement C3d and Factor B in relation to N3ECD in VSMCs from CADASIL subjects. A-F, Representative immunofluorescent images showing specific C3d (A, B) and Factor B (C, D) localization in VSMCs from control and CADASIL subjects. Specificity was also demonstrated by the localization of N3ECD reactivity in VSMCs in CADASIL but not in control VSMCs. Arrows show positive reactivity in VSMCs in CADASIL. Quantification showed that C3d and Factor B immunoreactivites were localised in > 70% of the cells (n = 3 wells). There was no apparent co-localisation of complement and N3ECD, which was always distally located [57]. Scale bar represents 15 μm (A-F)