Fig. 5
From: ER stress induced immunopathology involving complement in CADASIL: implications for therapeutics
qRT-PCR and Immunoblotting analyses of ICAM-1 and inflammatory genes in CADASIL VSMCs. A, Plots show expression of several inflammatory genes in VSMCs in CADASIL Patient-derived cerebral arterial VSMC (VSMCArg133Cys,n = 1) as well as control VSMC (VSMCWildeType,n = 1) cell line. Expression was normalized to the endogenous control gene; HPRT1, and the RQ (Relative Quantitation) was calculated using control VSMC normalized to 1. ** P < 0.05, **P < 0.01, ***P < 0.001. One-way ANOVA followed by Bonferroni’s post-hoc test was used for statistical analysis. B), Immunoblotting analyses were conducted to assess the protein expression of IL-6 and ICAM in VSMCs. The samples were visualized using the Odyssey CLx Imager. 15 µg of protein was loaded into the gel and GAPDH was used as loading control. C, Quantitative RT-PCR analysis of inflammatory gene expression in ECs co-cultured with CADASIL OR wildtype VSMC. Analysis showed increase in IL-12α and IL-1α gene expression in the ECs after they were co-cultured with Human Cerebral VSMC from CADASIL patient (*P < 0.05). The results are representative of three independent biological replicates (n = 3)