Fig. 2

Boosting NRG1-III expression in SMA mice increases VR myelinated axon number A Schematic of mouse breeding to generate genotypes: WT, WT NRG1-III + , SMA, and SMA NRG1-III + . B Western blot of NRG1-III protein expression in spinal cord protein lysates collected from WT, WT NRG1-III + , SMA, and SMA NRG1-III + mice at P10 (n = 3 each). C Representative toluidine blue–stained cross sections of L1 VRs in WT, WT NRG1-III + , SMA, and SMA NRG1-III + of mice at P2 (scale bar: 20 μm). The black arrow indicates a myelinated axon and the orange arrow indicates a Schwann cell nucleus. D Cross-sectional area, E myelinated axon number and F Schwann cell number in L1 VRs from WT (n = 3), WT NRG1-III + (n = 3), SMA (n = 6), and SMA NRG1-III + (n = 5) mice at P2. Data represent means and SEM. Statistical analysis was performed D–F, using one-way ANOVA. Significance of WT compared to SMA NRG1-III + and WT NRG1-III + compared to SMA not included. Significance: *p \(\le\) .05, **p \(<\) 0.01, ***p \(<\) 0.001