Table 3 List of primers used for quantitating mRNA expression using RT-qPCR
Gene Name | Forward/Reverse (5’-3’) | Sequence |
|---|---|---|
β-actin | Forward | CCAGCCTTCCTTCTTGGGTA |
Reverse | GAGGTCTTTACGGATGTCAACG | |
Monoaminergic transmission | ||
Slc6a4 | Forward | CAAAACGTCTGGCAAGGTGG |
Reverse | ACACCCCTGTCTCCAAGAGT | |
Tph2 | Forward | GACCCAAAGACGACCTGCTT |
Reverse | CTGCGTGTAGGGGTTGAAGT | |
Ido1 | Forward | GTATGTGTGGAACCGAGGGG |
Reverse | TCCAGTTTGCCAGGACACAG | |
Th | Forward | TACTTTGTGCGCTTCGAGGT |
Reverse | GGAACCTTGTCCTCTCTGGC | |
Maoa | Forward | GTATGTGAGGCAGTGTGGAGG |
Reverse | CCCCAAGGAGGACCATTATCTG | |
Maob | Forward | ATTCCACCTGCTTTGGGCAT |
Reverse | TGAACCCAAAGGCACACGA | |
Slc6a3 | Forward | AAAATGGTGGAGGTGTGGGC |
Reverse | GCTAGAGTTGCTGCTATGTGC | |
Slc10a4 | Forward | TCGTGAAGGTTTCCCTGTGG |
Reverse | ATGGCGACCACGTAAACAGT | |
Slc18a2 | Forward | GGACCACAACTGCCCCATTA |
Reverse | CGTTAGAGGGGCTCAGTCAC | |
5-HT receptors | ||
Htr1a | Forward | TACTCCACTTTCGGCGCTTT |
Reverse | GGCTGACCATTCAGGCTCTT | |
Htr1b | Forward | ACCCTTCTTCTGGCGTCAAG |
Reverse | ACCGTGGAGTAGACCGTGT | |
Htr2a | Forward | ACCGACATGCCTCTCCATTC |
Reverse | TGACCAGTATGTTTCCCGCA | |
Htr2c | Forward | GTGCCCGTTTTTCATCACCAA |
Reverse | AGGAGGCTTTTTGTCTGGCTT | |
Htr3a | Forward | CCATCTTCATTGTGCGGCTG |
Reverse | CTTGTTGGCTTGGAAGGTGG | |
Htr4 | Forward | GGAGTGTGCCAGGAGATCAG |
Reverse | AACCACTGCAAGGAACGTGA | |
Htr6 | Forward | AGTGGGAGGTGGTAGGTCTC |
Reverse | GGGCTGAGGACTGATTGCTT | |
Htr7 | Forward | AAGTTCTCAGGCTTCCCACG |
Reverse | TTCGCACACTCTTCCACCTC | |
Neuroinflammation | ||
Il1a | Forward | TTGCTGAAGGAGTTGCCAGA |
Reverse | GCACCCGACTTTGTTCTTTGG | |
Il1b | Forward | GCCACCTTTTGACAGTGATGAG |
Reverse | AAGGTCCACGGGAAAGACAC | |
Il6 | Forward | GAGACTTCCATCCAGTTGCCT |
Reverse | TCCTCTGTGAAGTCTCCTCTCC | |
Tnfrsf1a | Forward | AGCCACACCCACAACCTTAG |
Reverse | CCCCTTAGAGACCTTTGCCC | |
Il1r1 | Forward | ACTTGAGGAGGCAGTTTTCGT |
Reverse | GTCAATCTCCAGCGACAGCA | |
Il1r2 | Forward | ATCTTGGTTGTGGGGGCAAT |
Reverse | CCTGGTTGTCAGTCCGTAGC | |
Il2ra | Forward | TGAAGTGTGGGAAAACGGGG |
Reverse | GCAGGAAGTCTCACTCTCGG | |
Il10ra | Forward | GCGTGACTCTGAAAGCAATGG |
Reverse | GCAGCACCTTGACACAAAACT | |
Cx3cl1 | Forward | GAGAGTGAGGAAGCCAACCC |
Reverse | AAAGTCCGATGACGGGTGTC | |
Cx3cr1 | Forward | GGGTTTGGTGAGTCCTGGTT |
Reverse | CAAGGAATGGACACCCGACA | |
Protein aggregation | ||
App | Forward | TTCGCTGACGGAAACCAAGA |
Reverse | TTTCGGTATTGGCTGGCACA | |
Psen1 | Forward | CTCCTGCTCGCCATTTTCAAG |
Reverse | CACAAGGTAATCCGTGGCGA | |
Psen2 | Forward | ACACTGAGAAGAACGGGCAG |
Reverse | AGGAGCATCAGGGAGGACAT | |
Nesp55 | Forward | CCCGAGCAAGAACCTTTGGA |
Reverse | ACGGGCTCATTGTTAGACGG | |
Hsf1 | Forward | AGAGGAAAGTGACCAGCGTG |
Reverse | ACAACTTTTTGCTGCTGGGC | |
Gskip | Forward | GTTCGCCATTCCTTCACACG |
Reverse | GCAGCATTGAAGCCCTGTAAC | |
Tgm2 | Forward | AAGAGAAACTGGTGCTGCGT |
Reverse | GCACTGAGGCTGACCAAGAT | |
Kinases | ||
Frk | Forward | AGCAGGTCAGGAAGAAGCAC |
Reverse | CTCACCATACCTCCCGCTTC | |
Fyn | Forward | CAGCAAGACAAGGTGCGAAG |
Reverse | CCTGGGTATGGCACTCTTCC | |
Erk2 | Forward | AAGACACAGCACCTCAGCAA |
Reverse | GTGTTCAGCAGGAGGTTGGA | |
Gsk3b | Forward | GGAGTGAAAAGCCAAGAGAACG |
Reverse | CAAAGGAGGTGGTTCTCGGT | |
Csnk1a1 | Forward | CACAGGCAAGCAAACTGACAA |
Reverse | AACAAACGCTGCTCCAATCG | |
Csnk2a2 | Forward | AAGGAGCCATTCTTCCACGG |
Reverse | TCCGTGAATGTTGTCCCAGG | |
Prkacb | Forward | CAAGAAAGGCAGCGAAGTGG |
Reverse | ATTACTCGGGGGAGGGTTCT | |
Glutamatergic and Gabaergic transmission | ||
Gad1 | Forward | CCTTGAACCGTAGAGACCCC |
Reverse | TCAGGCCCAGTTTTCTGGTG | |
Gad2 | Forward | ACCGTGTATGGGGCTTTTGA |
Reverse | ATCAGTAACCCTCCACCCCA | |
Gls | Forward | ACTGTAGATGGGCAAAGGCA |
Reverse | AATCCACTTGGCTCCTTCCC | |
Grin1 | Forward | CTTCAGTCCCTTTGGCCGAT |
Reverse | ATGCCAGAGTTGAGCAGGAC | |
Grin2a | Forward | AGCTTGAAAACTGGGAAGTTGG |
Reverse | AGATGTACCCGCTCCCAATG | |
Grin2b | Forward | CGACCTGTACGGCAAGTTCT |
Reverse | TTGCTGCTTCCTCCTCTTGG | |