Table 1 Antibodies used in immunohistochemistry
Stain | Antibody | Concentration |
|---|---|---|
5HT-AT8 | Goat anti-5HT (Immunostar; catalog # 20079) | 1:2000 |
Mouse anti-AT8 (Thermofisher, catalog # MN1020) | 1:200 | |
405 Donkey anti-Goat (Jackson ImmunoResearch; catalog # 715-475-003) | 1:500 | |
Cy3 Donkey anti-Mouse (Jackson ImmunoResearch; catalog # 711-165-151) | 1:500 | |
TH-AT8 | Rabbit anti-TH (Novus; catalog # NB300-109) | 1:1000 |
Mouse anti-AT8 (Thermofisher, catalog # MN1020) | 1:200 | |
405 Donkey anti-Rabbit (Abcam; catalog # ab175651) | 1:500 | |
Cy3 Donkey anti-Mouse (Jackson ImmunoResearch; catalog # 715-165-151) | 1:500 | |
5HT-iba1-GFAP | Goat anti-5HT (Immunostar; catalog # 20079) | 1:2000 |
Rabbit anti-iba1 (Abcam; catalog # ab178846) | 1:200 | |
Chicken anti-GFAP (Abcam; catalog # ab4674) | 1:6000 | |
405 Donkey anti-Goat (Jackson ImmunoResearch; catalog # 715-475-003) | 1:500 | |
Cy3 Donkey anti-Rabbit (Jackson ImmunoResearch; catalog # 711-165-152) | 1:500 | |
647 Donkey anti-Chicken (Jackson ImmunoResearch; catalog # 703-605-155) | 1:500 | |
TH-iba1-GFAP | Mouse anti-TH (Millipore Sigma; catalog # MAB318) | 1:200 |
Rabbit anti-iba1 (Abcam; catalog # ab178846) | 1:200 | |
Chicken anti-GFAP (Abcam; catalog # ab4674) | 1:6000 | |
405 Donkey anti-Mouse (Invitrogen; catalog # A48257) | 1:500 | |
Cy3 Donkey anti-Rabbit (Jackson ImmunoResearch; catalog # 711-165-152) | 1:500 | |
647 Donkey anti-Chicken (Jackson ImmunoResearch; catalog # 703-605-155) | 1:500 | |
SERT | Rabbit anti-SERT (Immunostar; catalog #24330) | 1:2500 |
Alexa Fluor 555 donkey anti-rabbit (Invitrogen; catalog # A31572) | 1:500 |