Fig. 2
Hyperphosphorylated tau and monoaminergic depletion in the brainstem of male htau mice at 4 months. A Representative confocal images of 5-HT immunostaining (20X; scale bar = 200 µm) and 5-HT/AT8 co-staining (60X; scale bar = 50 µm) in the DRN of C57BL/6 J and htau +/- mice. B Atlas plate depicting one of the DRN regions analyzed (mid DRN; shown in blue) C Representative orthogonal image showing colocalization of ptau (AT8) with 5-HT neurons in the DRN (100X; scale bar = 20 µm). D Histogram of 5-HT cell counts/mm2, E % change in the 5-HT optical density, F % change in the ptau (AT8) optical density in subregions of the DRN and G Correlation analysis between 5-HT neuronal density and AT8 optical density in the DRN H Representative confocal images of TH immunostaining (20X; scale bar = 200 µm) and TH/AT8 colocalization (60X; scale bar = 50 µm) in the LC of C57BL/6 J and htau +/- mice. I Atlas plate depicting one of the LC regions analyzed (mid LC; shown in blue) J Representative orthogonal image showing colocalization of ptau with TH neurons in the LC (100X; scale bar = 20 µm). K Histogram of TH cell counts/mm2, L TH immunoreactive area, M ptau (AT8) optical density in subregions of the LC, and N Correlation analysis between TH immunoreactive area and AT8 optical density in the mid LC. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001