Fig. 3

Oligodendrocyte lineage responses in poorly myelinating developing white matter. A Mean oligodendrocyte lineage cell (OLIG2 +) number per mm² in control (n = 7 infants) and injured (n = 8 infants) cases. Unpaired 2-tailed Student’s t-test, **P = 0.0044. B OLIG2 + cells per mm² in control (green) and injured (pink) infants versus age (days old), in infants born at term. N = 7 infants per group. C OLIG2 + cells per mm² in cROI (n = 7 infants), iROI-M⁺ (n = 5 infants), versus iROI-M⁻ (n = 8 infants). Unpaired 2-tailed Student’s t-test, **P = 0.0028 for cROI versus iROI-M⁻, *P = 0.0423 for cROI versus iROI-M⁺. D Representative images of OLIG2 + cells (green) in cROI, iROI-M⁺, and iROI-M⁻, indicated by arrowheads. Nuclei counterstained with Hoechst (blue). Scale bar, 20 µm. E Mean CNPase + cells per mm² in iROI-M⁺ versus iROI-M⁻. Paired 2-tailed Student’s t-test, **P = 0.0046. n = 5 infants. F Representative images of CNPase + cells (red) in iROI-M⁺ and in iROI-M⁻, showing reduced and bleb-like processes in the latter. Nuclei counterstained with Hoechst (blue). Cell bodies indicated by asterisks. Scale bar, 20 µm. G Percentage of CNPase + area in iROI-M⁺ versus iROI-M⁻ in term infants (excluding pre-term Infant 2). Paired 2-tailed Student’s t-test, *P = 0.0478, n = 3 infants. H CNPase Disruption as measured by sphericity in iROI-M⁺ versus iROI-M⁻ in term infants. Paired 2-tailed Student’s t-test, *P = 0.0215, n = 4 infants