Fig. 3

Digital spatial profiling of BM-LUAD and BCBM. a Volcano plot of differential expression of proteins in immune infiltrate regions (CD45 + area) between BCBM and BM-LUAD (absolute fold change ≥ 1 and adj. p ≤ 0.05). b Box plots of protein targets in immune infiltrate compartments between BCBM and BM-LUAD. Each dot is presenting one CD45 + ROI in a BM sample. (* adj. p ≤ 0.05; ** adj. p ≤ 0.01). c Heatmap of median normalized protein expression in CD45 + ROIs between BCBM and BM-LUAD. d Heatmap of normalized protein expression in CD45 + ROIs between BCBM and BM-LUAD. The scaling of the heatmap is based on each target. (e) Image of a CD45 + ROI in BCBM sample. The bar graph shows the distribution of immune cell types in seven immune ROIs from BCBM. Image of a CD45 + ROI in BM-LUAD sample. f The bar graph shows the distribution of immune cell types in twenty-three immune ROIs from BM-LUAD