Fig. 2

Digital spatial profiling of BM-LUAD and BCBM. a Image of a brain metastasis tissue sample used for DSP analysis and stained with morphological markers DNA (nucleus, blue), PanCK (tumor, green), and CD45 (immune cells, pink). b Volcano plot of differential expression of proteins in PanCK + tumor regions between BCBM and BM-LUAD (absolute fold change ≥ 1 and adj. p ≤ 0.05). c Box plots of protein targets in tumor cells containing compartments between BCBM and BM-LUAD. Each dot is presenting one PanCK + ROI in a BM sample, each ROI was measured in three replicates in all the samples. d Heatmap of normalized protein expression in PanCK + tumor ROIs between BCBM and BM-LUAD. The scaling of the heatmap is based on each target. e Heatmap of median normalized protein expression in tumor cell containing compartments (PanCK + ROIs) between BCBM and BM-LUAD