Fig. 7

Identifying of microglia/macrophage phenotypes in human FFPE brain tissue using QUIVER. (A) Microglia/macrophages were grouped into one of five phenotypes based on unique marker expression. Human gray matter was analyzed to determine how many cells showed those phenotypes on average using the HALO object colocalization algorithm. (B–F) The pseudo-fluorescent images were created from deconvolved single-channel IHC images. IBA1 (yellow), P2Y12 (green), ferritin (blue), and CD68 (magenta) were included in the pseudo-fluorescent images. The white box indicates a cell that expressed the different marker classes, as determined by the HALO object colocalization algorithm. The other cells in the micrograph may not share the same cell phenotype. The original brightfield image of the IBA1 IHC is shown for the cell highlighted in the box to highlight differences and similarities in the IBA1⁺ cellular morphology among the molecular distinct microglia/macrophage phenotypes. Scale bar = 25 μm