Fig. 5

Comparison of single and sequential histological staining in glia-associated markers. Representative photomicrographs in similar regions from neighboring FFPE sections of the same sMTG tissue block, show comparative staining for the selected glial-associated stains. During each round of staining, tissue was stained alone as a positive control and sequentially using the multiplex staining method to show there is little to-no loss in antigenicity or stainability in subsequent rounds of staining. The difference in the area quantification of staining between the multiplex vs. single stain was 0.5% or less. The single stain and multiplex stain analysis was done on serial sections of the same sMTG tissue block; however, there may be a separation of up to 100 μm in the z dimension between sections. Photomicrographs were captured at × 10 magnification. Scalebar is 50 μm