Fig. 4

Panel of antibodies used from mIHC. FFPE human brain tissue was stained with antibodies used for the mIHC. The primary stain (1°) is shown, along with the image of the same section following the MICSSS de-staining, and then re-staining omitting the primary antibody. The order of antibody from left to right shows the order used on the mIHC panel. Using a HALO Area Quantification algorithm across the entire tissue section found, less than 0.03% of the primary stain, or other background noise, was detected in the de-stained and redeveloped tissue for all markers. Scale bar = 50 μm