Fig. 5
From: Loss of MBNL1-mediated retrograde BDNF signaling in the myotonic dystrophy brain
Impaired retrograde transport of BDNF-TrkB signaling in human postmortem DM1 brain. (a) Examination of cytoplasmic MBNL1 and dendrite integrity in the cerebral cortex of individual with DM1. Neuron expressing MBNL1 was marked with arrowhead. Fragmented dendrites are indicated by arrows (a7). (a8) Enlarged image from the inset in a7 shows the colocalization of MBNL1 with RNA foci in the nucleus. (b) Examination of p-TrkB and DYNLL2 levels in the P2 fraction from postmortem brain tissues of normal and DM1 individuals. HSP90 was detected as a loading control. (c) Representative TrkB immunoreactivity in the cell body of cortical neurons in DM1 brain. Number of human brain (N) and number of neurons (n) in each group is indicated. Data are presented as mean ± SEM. *p < 0.05, **p < 0.01, ***P < 0.001 by Student’s t-test. Scale: a1-7, 50 μm; a8, 10 μm; c, 20 μm